› 7md5

Electron Microscopy
5.2Å resolution

Insulin receptor ectodomain dimer complexed with two IRPA-9 partial agonists

Released:
DOI: 10.2210/pdb7md5/pdb
PDB entry 7md5 coloured by chain, front view.
PDB entry 7md5 coloured by chain, side view.
PDB entry 7md5 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Functionally selective signaling and broad metabolic benefits by novel insulin receptor partial agonists.
Wu M, Carballo-Jane E, Zhou H, Zafian P, Dai G, Liu M, Lao J, Kelly T, Shao D, Gorski J, Pissarnitski D, Kekec A, Chen Y, Previs SF, Scapin G, Llorente YG, Hollingsworth SA, Yan L, Feng D, Huo P, Walford G, Erion MD, Kelley DE, Lin S, Mu J
OpenAccess logo Nat Commun 13 942 (2022)
PMID: 35177603
Related structures: EMD-23767

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134231 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (8 distinct):
Insulin receptor subunit alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 927 amino acids
Theoretical weight: 106.14 KDa
Source organism: Homo sapiens
Expression system: Mus musculus
UniProt:
  • Canonical: P06213 (Residues: 28-956; Coverage: 68%)
  • Best match: P06213-2 (Residues: 28-944)
Gene name: INSR
Sequence domains:
Insulin receptor subunit alpha Chains: M, N
Molecule details ›
Chains: M, N
Length: 30 amino acids
Theoretical weight: 3.46 KDa
Source organism: Homo sapiens
Expression system: Mus musculus
UniProt:
  • Canonical: P06213 (Residues: 721-746; Coverage: 2%)
  • Best match: P06213-2 (Residues: 721-747)
Gene name: INSR
Insulin A chain Chains: O, Q, S, U
Molecule details ›
Chains: O, Q, S, U
Length: 21 amino acids
Theoretical weight: 2.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01308 (Residues: 90-110; Coverage: 24%)
Gene name: INS
Insulin B chain Chains: P, R, T, V
Molecule details ›
Chains: P, R, T, V
Length: 30 amino acids
Theoretical weight: 3.43 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01308 (Residues: 25-54; Coverage: 35%)
Gene name: INS
Sequence domains: Insulin/IGF/Relaxin family

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, FUC
Ligand NAG 8 x NAG
Ligand FUC 4 x FUC
Carbohydrate polymer : NEW Components: NAG, BMA
Ligand NAG 4 x NAG
Ligand BMA 2 x BMA
Carbohydrate polymer : NEW Components: NAG
Ligand NAG 4 x NAG
Carbohydrate polymer : NEW Components: NAG, FUC
Ligand NAG 2 x NAG
Ligand FUC 2 x FUC
1 bound ligand:
Ligand NAG 4 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 5.2Å
Relevant EMDB volumes: EMD-23767
Expression systems:
  • Mus musculus
  • Escherichia coli

Quick links

Citations

1 review citation

A stepwise activation model for the insulin receptor.
Yunn et al. (2023)
5 other articles

1 mention without citation

Functionally selective signaling and broad metabolic benefits by novel insulin receptor partial agonists.
Wu et al. (2022)