› 7mer

X-ray diffraction
1.74Å resolution

Structure of ALDH4A1 complexed with trans-4-Hydroxy-L-proline

Released:
DOI: 10.2210/pdb7mer/pdb
PDB entry 7mer coloured by chain, front view.
PDB entry 7mer coloured by chain, side view.
PDB entry 7mer coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural basis for the stereospecific inhibition of the dual proline/hydroxyproline catabolic enzyme ALDH4A1 by trans-4-hydroxy-L-proline.
Bogner AN, Stiers KM, McKay CM, Becker DF, Tanner JJ
Protein Sci 30 1714-1722 (2021)
PMID: 34048122

Function and Biology Details

Reaction catalysed: 
L-glutamate 5-semialdehyde + NAD(+) + H(2)O = L-glutamate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183972 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 563 amino acids
Theoretical weight: 61.95 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8CHT0 (Residues: 21-562; Coverage: 96%)
Gene name: Aldh4a1
Sequence domains: Aldehyde dehydrogenase family

Ligands and Environments

4 bound ligands:
Ligand HYP 2 x HYP
Ligand 1PE 2 x 1PE
Ligand SO4 2 x SO4
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P212121
Unit cell:
a: 85.056Å b: 94.6Å c: 132.587Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.164 0.19
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Aldehyde Dehydrogenase 2 as a Therapeutic Target in Oxidative Stress-Related Diseases: Post-Translational Modifications Deserve More Attention.
Gao et al. (2022)
2 other articles

69 mentions without citation

Identifying miRNAs, targets and functions.
Liu et al. (2014)
68 more