Function and Biology Details
Reactions catalysed:
L-glutamate + H(2)O + NAD(+) = 2-oxoglutarate + NH(3) + NADH
(1a) L-glutamate + NH(3) = L-glutamine + H(2)O
Biochemical function:
Biological process:
Cellular component:
- not assigned
Sequence domains:
- Glutamate/phenylalanine/leucine/valine/L-tryptophan dehydrogenase
- FAD/NAD(P)-binding domain superfamily
- Glutamate synthase, alpha subunit, C-terminal
- Glutamate synthase, alpha subunit, C-terminal domain superfamily
- Glutamate synthase domain
- Aldolase-type TIM barrel
- Nucleophile aminohydrolases, N-terminal
- Glutamine amidotransferase type 2 domain
12 more domains
Structure analysis Details
Assembly composition:
hetero octadecamer (preferred)
PDBe Complex ID:
PDB-CPX-128536 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Cryptic catabolic NAD-specific glutamate dehydrogenase GudB
Molecule details ›
Chain: A
Length: 424 amino acids
Theoretical weight: 47.1 KDa
Source organism: Bacillus subtilis subsp. subtilis NCIB 3610 = ATCC 6051 = DSM 10
Expression system: Bacillus subtilis
UniProt:
Sequence domains:
Length: 424 amino acids
Theoretical weight: 47.1 KDa
Source organism: Bacillus subtilis subsp. subtilis NCIB 3610 = ATCC 6051 = DSM 10
Expression system: Bacillus subtilis
UniProt:
- Canonical:
P50735 (Residues: 1-427; Coverage: 99%)
Sequence domains:
Glutamate synthase [NADPH] large chain
Molecule details ›
Chain: G
Length: 1520 amino acids
Theoretical weight: 169.01 KDa
Source organism: Bacillus subtilis subsp. subtilis NCIB 3610 = ATCC 6051 = DSM 10
Expression system: Bacillus subtilis
UniProt:
Sequence domains:
Length: 1520 amino acids
Theoretical weight: 169.01 KDa
Source organism: Bacillus subtilis subsp. subtilis NCIB 3610 = ATCC 6051 = DSM 10
Expression system: Bacillus subtilis
UniProt:
- Canonical: P39812 (Residues: 1-1520; Coverage: 100%)
Sequence domains:
Glutamate synthase [NADPH] small chain
Molecule details ›
Chain: I
Length: 524 amino acids
Theoretical weight: 58.01 KDa
Source organism: Bacillus subtilis subsp. subtilis NCIB 3610 = ATCC 6051 = DSM 10
Expression system: Bacillus subtilis
UniProt:
Sequence domains:
Length: 524 amino acids
Theoretical weight: 58.01 KDa
Source organism: Bacillus subtilis subsp. subtilis NCIB 3610 = ATCC 6051 = DSM 10
Expression system: Bacillus subtilis
UniProt:
- Canonical: O34399 (Residues: 1-493; Coverage: 100%)
Sequence domains:
