› 7mg8

X-ray diffraction
3Å resolution

Concanavalin A bound to a DNA glycoconjugate, Man-CGCG

Released:
DOI: 10.2210/pdb7mg8/pdb
PDB entry 7mg8 coloured by chain, front view.
PDB entry 7mg8 coloured by chain, side view.
PDB entry 7mg8 coloured by chain, top view.
Source organisms:
Primary publication:
Redefining Protein Interfaces within Protein Single Crystals with DNA.
Partridge BE, Winegar PH, Han Z, Mirkin CA
J Am Chem Soc 143 8925-8934 (2021)
PMID: 34096291

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-511755 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Concanavalin-A, 2nd part Chains: A, B
Molecule details ›
Chains: A, B
Length: 237 amino acids
Theoretical weight: 25.62 KDa
Source organism: Canavalia ensiformis
UniProt:
  • Canonical: P02866 (Residues: 28-148; Coverage: 46%)
Sequence domains: Legume lectin domain
DNA (5'-D(P*CP*GP*CP*G)-3') Chains: C, D
Molecule details ›
Chains: C, D
Length: 4 nucleotides
Theoretical weight: 1.19 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

4 bound ligands:
Ligand MN 2 x MN
Ligand CA 2 x CA
Ligand MAN 2 x MAN
Ligand SQ0 2 x SQ0
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Unit cell:
a: 66.419Å b: 69.92Å c: 125.869Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.236 0.233 0.284
Expression system: Not provided

Quick links

Citations

1 review citation

Protein Assembly by Design.
Zhu et al. (2021)
2 other articles

1 mention without citation

Redefining Protein Interfaces within Protein Single Crystals with DNA.
Partridge et al. (2021)