› 7mg9

X-ray diffraction
2.55Å resolution

Concanavalin A bound to DNA glycoconjugates, Man-TTTT and Man-AAAA

Released:
DOI: 10.2210/pdb7mg9/pdb
PDB entry 7mg9 coloured by chain, front view.
PDB entry 7mg9 coloured by chain, side view.
PDB entry 7mg9 coloured by chain, top view.
Source organisms:
Primary publication:
Redefining Protein Interfaces within Protein Single Crystals with DNA.
Partridge BE, Winegar PH, Han Z, Mirkin CA
J Am Chem Soc 143 8925-8934 (2021)
PMID: 34096291

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-121629 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Concanavalin-A, 2nd part Chains: A, B
Molecule details ›
Chains: A, B
Length: 237 amino acids
Theoretical weight: 25.62 KDa
Source organism: Canavalia ensiformis
UniProt:
  • Canonical: P02866 (Residues: 28-148; Coverage: 46%)
Sequence domains: Legume lectin domain
DNA (5'-D(P*AP*AP*AP*A)-3') Chain: C
Molecule details ›
Chain: C
Length: 4 nucleotides
Theoretical weight: 1.21 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(P*TP*TP*TP*T)-3') Chain: D
Molecule details ›
Chain: D
Length: 4 nucleotides
Theoretical weight: 1.17 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

4 bound ligands:
Ligand MN 2 x MN
Ligand CA 2 x CA
Ligand MAN 2 x MAN
Ligand SQ0 2 x SQ0
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Unit cell:
a: 65.979Å b: 70.18Å c: 124.42Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.253 0.25 0.314
Expression system: Not provided

Quick links

Citations

1 review citation

Protein Assembly by Design.
Zhu et al. (2021)
2 other articles

1 mention without citation

Redefining Protein Interfaces within Protein Single Crystals with DNA.
Partridge et al. (2021)