Function and Biology Details
Reaction catalysed:
Exonucleolytic cleavage in the 5'- to 3'-direction to yield nucleoside 3'-phosphates
Biochemical function:
Biological process:
Cellular component:
- not assigned
Sequence domains:
- CRISPR-associated protein Cas1
- CRISPR-associated endonuclease Cas2
- CRISPR-associated endonuclease Cas1, C-terminal domain
- CRISPR-associated endonuclease Cas1, N-terminal domain
- Dna2/Cas4, domain of unknown function DUF83
- CRISPR-associated protein Cas4
- PD-(D/E)XK endonuclease-like domain superfamily
- Virulence-associated protein D / CRISPR associated protein Cas2
Structure analysis Details
Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-121633 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
CRISPR-associated exonuclease Cas4/endonuclease Cas1 fusion
Molecule details ›
Chains: A, B, C, D
Length: 559 amino acids
Theoretical weight: 62.6 KDa
Source organism: Geobacter sulfurreducens PCA
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains:
Length: 559 amino acids
Theoretical weight: 62.6 KDa
Source organism: Geobacter sulfurreducens PCA
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
Q74H36 (Residues: 1-559; Coverage: 100%)
Sequence domains:
CRISPR-associated endoribonuclease Cas2
Molecule details ›
Chains: E, F
Length: 95 amino acids
Theoretical weight: 11.19 KDa
Source organism: Geobacter sulfurreducens PCA
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: CRISPR associated protein Cas2
Length: 95 amino acids
Theoretical weight: 11.19 KDa
Source organism: Geobacter sulfurreducens PCA
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: Q74H35 (Residues: 1-95; Coverage: 100%)
Sequence domains: CRISPR associated protein Cas2
