› 7mii

Electron Microscopy
2.7Å resolution

Human CTPS2 bound to inhibitor T35

Released:
DOI: 10.2210/pdb7mii/pdb
PDB entry 7mii coloured by chain, front view.
PDB entry 7mii coloured by chain, side view.
PDB entry 7mii coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for isoform-specific inhibition of human CTPS1.
Lynch EM, DiMattia MA, Albanese S, van Zundert GCP, Hansen JM, Quispe JD, Kennedy MA, Verras A, Borrelli K, Toms AV, Kaila N, Kreutter KD, McElwee JJ, Kollman JM
OpenAccess logo Proc Natl Acad Sci U S A 118 (2021)
PMID: 34583994
Related structures: EMD-23852

Function and Biology Details

Reaction catalysed: 
(1a) L-glutamine + H(2)O = L-glutamate + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192557 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CTP synthase 2 Chains: A, B, C, E
Molecule details ›
Chains: A, B, C, E
Length: 586 amino acids
Theoretical weight: 65.76 KDa
Source organism: Homo sapiens
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q9NRF8 (Residues: 1-586; Coverage: 100%)
Gene name: CTPS2
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand ZFY 4 x ZFY
Ligand UTP 4 x UTP
Ligand GLN 4 x GLN
Ligand MG 4 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.7Å
Relevant EMDB volumes: EMD-23852
Expression system: Saccharomyces cerevisiae

Quick links

Citations

3 review citations

Re-Discovery of Pyrimidine Salvage as Target in Cancer Therapy.
Walter et al. (2022)
2 more