› 7mkv

X-ray diffraction
2.25Å resolution

Engineered PLP-dependent decarboxylative aldolase from Aspergillus flavus, UstD2.0, bound as the internal aldimine

Released:
DOI: 10.2210/pdb7mkv/pdb
PDB entry 7mkv coloured by chain, front view.
PDB entry 7mkv coloured by chain, side view.
PDB entry 7mkv coloured by chain, top view.
Source organism: Aspergillus flavus
Primary publication:
Biocatalytic synthesis of non-standard amino acids by a decarboxylative aldol reaction
Ellis JM, Campbell ME, Kumar P, Geunes EP, Bingman CA, Buller AR
Nat Catal 5 136-143 (2022)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110751 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cysteine desulfurase-like protein ustD Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 447 amino acids
Theoretical weight: 49.6 KDa
Source organism: Aspergillus flavus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B8NM72 (Residues: 1-439; Coverage: 100%)
Gene names: AFLA_095040, ustD
Sequence domains: Aminotransferase class-V

Ligands and Environments

No bound ligands
2 modified residues:
Ligand CSD 3 x CSD
Ligand LLP 3 x LLP

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: I422
Unit cell:
a: 162.09Å b: 162.09Å c: 221.005Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.214 0.246
Expression system: Escherichia coli BL21(DE3)

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