› 7mlv

Electron Microscopy
4.1Å resolution

Cryo-EM reveals partially and fully assembled native glycine receptors,homomeric tetramer

Released:
DOI: 10.2210/pdb7mlv/pdb
PDB entry 7mlv coloured by chain, front view.
PDB entry 7mlv coloured by chain, side view.
PDB entry 7mlv coloured by chain, top view.
Source organisms:
Primary publication:
Architecture and assembly mechanism of native glycine receptors.
Zhu H, Gouaux E
Nature 599 513-517 (2021)
PMID: 34555840
Related structures: EMD-23911

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-207844 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (6 distinct):
3D1 Fab Light Chain Chains: I, J, K, M
Molecule details ›
Chains: I, J, K, M
Length: 107 amino acids
Theoretical weight: 11.74 KDa
Source organism: Rattus norvegicus
Expression system: Rattus norvegicus
3D1 Fab Heavy Chain Chains: F, G, H, L
Molecule details ›
Chains: F, G, H, L
Length: 118 amino acids
Theoretical weight: 12.98 KDa
Source organism: Rattus norvegicus
Expression system: Rattus norvegicus
Glycine receptor alpha 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 456 amino acids
Theoretical weight: 52.64 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A4X1TUM3 (Residues: 1-456; Coverage: 100%)
Gene name: GLRA1
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: MAN, BMA, NAG
Ligand MAN 2 x MAN
Ligand BMA 1 x BMA
Ligand NAG 2 x NAG
Carbohydrate polymer : NEW Components: MAN, BMA, NAG
Ligand MAN 1 x MAN
Ligand BMA 1 x BMA
Ligand NAG 2 x NAG
Carbohydrate polymer : NEW Components: MAN, BMA, NAG
Ligand MAN 3 x MAN
Ligand BMA 1 x BMA
Ligand NAG 2 x NAG
3 bound ligands:
Ligand MAN 4 x MAN
Ligand BMA 1 x BMA
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.1Å
Relevant EMDB volumes: EMD-23911
Expression system: Rattus norvegicus

Quick links

Citations

8 review citations

Startle Disease: New Molecular Insights into an Old Neurological Disorder.
Schaefer et al. (2023)
7 more

2 mentions without citation

Structure and Mechanism of Glycine Receptor Elucidated by Cryo-Electron Microscopy.
Zhu H. (2022)
1 more