7mns

X-ray diffraction
2.1Å resolution

Crystal Structure of the ZnF4 of Nucleoporin NUP358/RanBP2 in complex with Ran-GDP

Released:
Source organism: Homo sapiens
Entry authors: Bley CJ, Nie S, Mobbs GW, Petrovic S, Gres AT, Liu X, Mukherjee S, Harvey S, Huber FM, Lin DH, Brown B, Tang AW, Rundlet EJ, Correia AR, Chen S, Regmi SG, Stevens TA, Jette CA, Dasso M, Patke A, Palazzo AF, Kossiakoff AA, Hoelz A

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-156036 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
GTP-binding nuclear protein Ran Chain: A
Molecule details ›
Chain: A
Length: 236 amino acids
Theoretical weight: 26.57 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P62826 (Residues: 1-216; Coverage: 100%)
Gene names: ARA24, OK/SW-cl.81, RAN
Sequence domains: Ras family
E3 SUMO-protein ligase RanBP2 Chain: B
Molecule details ›
Chain: B
Length: 43 amino acids
Theoretical weight: 4.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P49792 (Residues: 1535-1571; Coverage: 1%)
Gene names: NUP358, RANBP2
Sequence domains: Zn-finger in Ran binding protein and others

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P6522
Unit cell:
a: 92.48Å b: 92.48Å c: 194.78Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.176 0.175 0.202
Expression system: Escherichia coli