› 7mnt

X-ray diffraction
2.45Å resolution

Crystal Structure of the ZnF5 or ZnF6 of Nucleoporin NUP358/RanBP2 in complex with Ran-GDP

Released:
DOI: 10.2210/pdb7mnt/pdb
PDB entry 7mnt coloured by chain, front view.
PDB entry 7mnt coloured by chain, side view.
PDB entry 7mnt coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Bley CJ, Nie S, Mobbs GW, Petrovic S, Gres AT, Liu X, Mukherjee S, Harvey S, Huber FM, Lin DH, Brown B, Tang AW, Rundlet EJ, Correia AR, Chen S, Regmi SG, Stevens TA, Jette CA, Dasso M, Patke A, Palazzo AF, Kossiakoff AA, Hoelz A

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-546387 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
GTP-binding nuclear protein Ran Chains: A, C
Molecule details ›
Chains: A, C
Length: 236 amino acids
Theoretical weight: 26.57 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P62826 (Residues: 1-216; Coverage: 100%)
Gene names: ARA24, OK/SW-cl.81, RAN
Sequence domains: Ras family
E3 SUMO-protein ligase RanBP2 Chains: B, D
Molecule details ›
Chains: B, D
Length: 43 amino acids
Theoretical weight: 4.59 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P49792 (Residues: 1657-1693; Coverage: 1%)
Gene names: NUP358, RANBP2
Sequence domains: Zn-finger in Ran binding protein and others

Ligands and Environments

3 bound ligands:
Ligand GDP 2 x GDP
Ligand MG 2 x MG
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P212121
Unit cell:
a: 53.02Å b: 94.27Å c: 108.16Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.212 0.257
Expression system: Escherichia coli

Quick links

Citations

13 review citations

The Nuclear Pore Complex: Birth, Life, and Death of a Cellular Behemoth.
Dultz et al. (2022)
12 more