› 7mo0

X-ray diffraction
2.45Å resolution

Crystal Structure of Nucleoporin NUP50 Ran-Binding Domain in Complex with Ran-GPPNHP

Released:
DOI: 10.2210/pdb7mo0/pdb
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PDB entry 7mo0 coloured by chain, front view.
PDB entry 7mo0 coloured by chain, side view.
PDB entry 7mo0 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Bley CJ, Nie S, Mobbs GW, Petrovic S, Gres AT, Liu X, Mukherjee S, Harvey S, Huber FM, Lin DH, Brown B, Tang AW, Rundlet EJ, Correia AR, Chen S, Regmi SG, Stevens TA, Jette CA, Dasso M, Patke A, Palazzo AF, Kossiakoff AA, Hoelz A

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-280011 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
GTP-binding nuclear protein Ran Chains: A, C
Molecule details ›
Chains: A, C
Length: 217 amino acids
Theoretical weight: 24.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P62826 (Residues: 1-216; Coverage: 100%)
Gene names: ARA24, OK/SW-cl.81, RAN
Sequence domains: Ras family
Nuclear pore complex protein Nup50 Chains: B, D
Molecule details ›
Chains: B, D
Length: 134 amino acids
Theoretical weight: 15.22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UKX7 (Residues: 337-468; Coverage: 28%)
Gene names: NPAP60L, NUP50, PRO1146
Sequence domains: RanBP1 domain

Ligands and Environments

3 bound ligands:
Ligand GNP 2 x GNP
Ligand MG 2 x MG
Ligand SO4 1 x SO4
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P212121
Unit cell:
a: 66.59Å b: 73.17Å c: 152.94Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.212 0.239
Expression system: Escherichia coli

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Citations

21 review citations

The Nuclear Pore Complex: Birth, Life, and Death of a Cellular Behemoth.
Dultz et al. (2022)
20 more

1 mention without citation

Nanostructured Ti07Mo0₃O₂ as Efficient Non-Carbon Support for PtRu Catalysts in Direct Methanol Fuel Cells.
Thanh Ho et al. (2018)

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