7mpz

X-ray diffraction
2.04Å resolution

HNH Nuclease Domain from G. stearothermophilus Cas9

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-245382 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CRISPR-associated endonuclease Cas9 Chain: A
Molecule details ›
Chain: A
Length: 154 amino acids
Theoretical weight: 18.51 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-003
Spacegroup: P41212
Unit cell:
a: 57.956Å b: 57.956Å c: 119.393Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.194 0.238
Expression system: Escherichia coli BL21(DE3)