› 7mqs

Electron Microscopy
4.4Å resolution

The insulin receptor ectodomain in complex with three venom hybrid insulin molecules - asymmetric conformation

Released:
DOI: 10.2210/pdb7mqs/pdb
PDB entry 7mqs coloured by chain, front view.
PDB entry 7mqs coloured by chain, side view.
PDB entry 7mqs coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Symmetric and asymmetric receptor conformation continuum induced by a new insulin.
Xiong X, Blakely A, Kim JH, Menting JG, Schäfer IB, Schubert HL, Agrawal R, Gutmann T, Delaine C, Zhang YW, Artik GO, Merriman A, Eckert D, Lawrence MC, Coskun Ü, Fisher SJ, Forbes BE, Safavi-Hemami H, Hill CP, Chou DH
OpenAccess logo Nat Chem Biol 18 511-519 (2022)
PMID: 35289328
Related structures: EMD-23951

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134225 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Insulin receptor subunit alpha Chains: E, F
Molecule details ›
Chains: E, F
Length: 916 amino acids
Theoretical weight: 104.63 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P06213 (Residues: 28-955; Coverage: 68%)
  • Best match: P06213-2 (Residues: 28-943)
Gene name: INSR
Sequence domains:
Insulin A chain Chains: A, C, G
Molecule details ›
Chains: A, C, G
Length: 24 amino acids
Theoretical weight: 2.74 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01308 (Residues: 90-110; Coverage: 24%)
Gene name: INS
Insulin B chain Chains: B, D, H
Molecule details ›
Chains: B, D, H
Length: 22 amino acids
Theoretical weight: 2.54 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01308 (Residues: 25-46; Coverage: 26%)
Gene name: INS

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.4Å
Relevant EMDB volumes: EMD-23951
Expression systems:
  • Homo sapiens
  • Not provided

Quick links

Citations

1 review citation

A stepwise activation model for the insulin receptor.
Yunn et al. (2023)
8 other articles

1 mention without citation

Molecular basis for the role of disulfide-linked αCTs in the activation of insulin-like growth factor 1 receptor and insulin receptor.
Li et al. (2022)