› 7mse

X-ray diffraction
1.27Å resolution

High-resolution crystal structure of hMIF2 with tartrate at the active site

Released:
DOI: 10.2210/pdb7mse/pdb
PDB entry 7mse coloured by chain, front view.
PDB entry 7mse coloured by chain, side view.
PDB entry 7mse coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A structurally preserved allosteric site in the MIF superfamily affects enzymatic activity and CD74 activation in D-dopachrome tautomerase.
Chen E, Reiss K, Shah D, Manjula R, Allen B, Murphy EL, Murphy JW, Batista VS, Bhandari V, Lolis EJ, Lisi GP
OpenAccess logo J Biol Chem 297 101061 (2021)
PMID: 34384784

Function and Biology Details

Reaction catalysed: 
D-dopachrome = 5,6-dihydroxyindole + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151620 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D-dopachrome decarboxylase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 117 amino acids
Theoretical weight: 12.59 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P30046 (Residues: 2-118; Coverage: 99%)
Gene name: DDT
Sequence domains: Macrophage migration inhibitory factor (MIF)

Ligands and Environments

1 bound ligand:
Ligand TLA 3 x TLA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P3
Unit cell:
a: 83.396Å b: 83.396Å c: 40.429Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.139 0.137 0.173
Expression system: Escherichia coli

Quick links

Citations

1 review citation

OxMIF: a druggable isoform of macrophage migration inhibitory factor in cancer and inflammatory diseases.
Thiele et al. (2022)
4 other articles

3 mentions without citation

A structurally preserved allosteric site in the MIF superfamily affects enzymatic activity and CD74 activation in D-dopachrome tautomerase.
Chen et al. (2021)
2 more