› 7mw4

Electron Microscopy
3.42Å resolution

Structure of the SARS-CoV-2 Spike trimer with one RBD down in complex with the Fab fragment of human neutralizing antibody clone 6

Released:
DOI: 10.2210/pdb7mw4/pdb
PDB entry 7mw4 coloured by chain, front view.
PDB entry 7mw4 coloured by chain, side view.
PDB entry 7mw4 coloured by chain, top view.
Source organisms:
Primary publication:
Monospecific and bispecific monoclonal SARS-CoV-2 neutralizing antibodies that maintain potency against B.1.617.
Peng L, Hu Y, Mankowski MC, Ren P, Chen RE, Wei J, Zhao M, Li T, Tripler T, Ye L, Chow RD, Fang Z, Wu C, Dong MB, Cook M, Wang G, Clark P, Nelson B, Klein D, Sutton R, Diamond MS, Wilen CB, Xiong Y, Chen S
OpenAccess logo Nat Commun 13 1638 (2022)
PMID: 35347138
Related structures: EMD-24062

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-535364 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (5 distinct):
Spike glycoprotein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 1288 amino acids
Theoretical weight: 142.4 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: Mammalian expression vector pcDNA3.1-Flag
UniProt:
  • Canonical: P0DTC2 (Residues: 1-1208; Coverage: 95%)
Gene names: 2, S
Sequence domains:
Fab of antibody clone 6, light chain Chains: E, G, L
Molecule details ›
Chains: E, G, L
Length: 238 amino acids
Theoretical weight: 25.92 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Fab of antibody clone 6, heavy chain Chains: D, F, H
Molecule details ›
Chains: D, F, H
Length: 237 amino acids
Theoretical weight: 25.5 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand NAG 28 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.42Å
Relevant EMDB volumes: EMD-24062
Expression systems:
  • Mammalian expression vector pcDNA3.1-Flag
  • Homo sapiens

Quick links

Citations

2 review citations

Applications of single-cell RNA sequencing in drug discovery and development.
Van de Sande et al. (2023)
1 more

4 mentions without citation

Monospecific and bispecific monoclonal SARS-CoV-2 neutralizing antibodies that maintain potency against B.1.617.
Peng et al. (2021)
3 more