› 7mxi

X-ray diffraction
2.8Å resolution

IgE-Fc in complex with DARPins E2_79 and E3_53

Released:
DOI: 10.2210/pdb7mxi/pdb
PDB entry 7mxi coloured by chain, front view.
PDB entry 7mxi coloured by chain, side view.
PDB entry 7mxi coloured by chain, top view.
Source organisms:
Primary publication:
Structure-guided design of ultrapotent disruptive IgE inhibitors to rapidly terminate acute allergic reactions.
Pennington LF, Gasser P, Brigger D, Guntern P, Eggel A, Jardetzky TS
J Allergy Clin Immunol 148 1049-1060 (2021)
PMID: 33991582

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-525435 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (5 distinct):
Immunoglobulin heavy constant epsilon Chains: A, B
Molecule details ›
Chains: A, B
Length: 247 amino acids
Theoretical weight: 27.55 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P01854 (Residues: 209-426; Coverage: 40%)
Gene name: IGHE
Sequence domains: Immunoglobulin C1-set domain
DARPin E3_53 Chains: C, D
Molecule details ›
Chains: C, D
Length: 173 amino acids
Theoretical weight: 18.78 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Anti-IgE Inhibitor E2_79 Chains: E, F
Molecule details ›
Chains: E, F
Length: 143 amino acids
Theoretical weight: 15.53 KDa
Source organism: synthetic construct
Expression system: Escherichia coli

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P21
Unit cell:
a: 51.908Å b: 137.793Å c: 88.25Å
α: 90° β: 105.68° γ: 90°
R-values:
R R work R free
0.215 0.213 0.256
Expression systems:
  • Homo sapiens
  • Escherichia coli

Quick links

Citations

11 review citations

FcεRI: A Master Regulator of Mast Cell Functions.
Nagata et al. (2022)
10 more