› 7mxj

X-ray diffraction
1.92Å resolution

Crystal structure of the S/T protein kinase PknG from Corynebacterium glutamicum (residues 130-433) in complex with AMP-PNP, isoform 1

Released:
DOI: 10.2210/pdb7mxj/pdb
PDB entry 7mxj coloured by chain, front view.
PDB entry 7mxj coloured by chain, side view.
PDB entry 7mxj coloured by chain, top view.
Source organism: Corynebacterium glutamicum ATCC 13032
Primary publication:
A Tetratricopeptide Repeat Scaffold Couples Signal Detection to OdhI Phosphorylation in Metabolic Control by the Protein Kinase PknG.
Lisa MN, Sogues A, Barilone N, Baumgart M, Gil M, Graña M, Durán R, Biondi RM, Bellinzoni M, Bott M, Alzari PM
OpenAccess logo mBio 12 e0171721 (2021)
PMID: 34607462

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185598 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein kinase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 305 amino acids
Theoretical weight: 33.27 KDa
Source organism: Corynebacterium glutamicum ATCC 13032
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8NM29 (Residues: 59-362; Coverage: 41%)
Gene name: Cgl2751
Sequence domains: Protein kinase domain

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand MAP 1 x MAP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 37.616Å b: 55.936Å c: 123.945Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.199 0.225
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 citation in other articles

Characteristics of the GlnH and GlnX Signal Transduction Proteins Controlling PknG-Mediated Phosphorylation of OdhI and 2-Oxoglutarate Dehydrogenase Activity in Corynebacterium glutamicum.
Sundermeyer et al. (2022)
1 more

1 mention without citation

A Tetratricopeptide Repeat Scaffold Couples Signal Detection to OdhI Phosphorylation in Metabolic Control by the Protein Kinase PknG.
Lisa et al. (2021)