› 7myq

X-ray diffraction
2.55Å resolution

Crystal structure of a tRNA (guanine-N1)-methyltransferase from Acinetobacter baumannii

Released:
DOI: 10.2210/pdb7myq/pdb
PDB entry 7myq coloured by chain, front view.
PDB entry 7myq coloured by chain, side view.
PDB entry 7myq coloured by chain, top view.
Source organism: Acinetobacter baumannii
Entry authors: Seattle Structural Genomics Center for Infectious Disease, Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109229 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA (guanine-N(1)-)-methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 254 amino acids
Theoretical weight: 28.92 KDa
Source organism: Acinetobacter baumannii
Expression system: Escherichia coli
UniProt:
  • Canonical: B0V8J1 (Residues: 2-246; Coverage: 100%)
Gene names: ABAYE0323, trmD
Sequence domains: tRNA (Guanine-1)-methyltransferase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P41
Unit cell:
a: 66.25Å b: 66.25Å c: 133.01Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.193 0.229
Expression system: Escherichia coli

Quick links