› 7mz0

X-ray diffraction
2.02Å resolution

Structure of human DNA polymerase beta complexed with dzA as the template base in a 1-nucleotide gapped DNA

Released:
DOI: 10.2210/pdb7mz0/pdb
PDB entry 7mz0 coloured by chain, front view.
PDB entry 7mz0 coloured by chain, side view.
PDB entry 7mz0 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Koag M-C, Lee S

Function and Biology Details

Reactions catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-121664 (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct DNA molecules
Macromolecules (4 distinct):
DNA polymerase beta Chain: A
Molecule details ›
Chain: A
Length: 329 amino acids
Theoretical weight: 37.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P06746 (Residues: 7-335; Coverage: 98%)
Gene name: POLB
Sequence domains:
DNA (5'-D(*CP*CP*GP*AP*CP*(4DU)P*TP*CP*GP*CP*AP*TP*CP*AP*GP*C)-3') Chain: T
Molecule details ›
Chain: T
Length: 16 nucleotides
Theoretical weight: 4.83 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*GP*CP*TP*GP*AP*TP*GP*CP*GP*A)-3') Chain: P
Molecule details ›
Chain: P
Length: 10 nucleotides
Theoretical weight: 3.09 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(P*GP*TP*CP*GP*G)-3') Chain: D
Molecule details ›
Chain: D
Length: 5 nucleotides
Theoretical weight: 1.54 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.3
Spacegroup: P21
Unit cell:
a: 54.649Å b: 79.172Å c: 54.819Å
α: 90° β: 106.27° γ: 90°
R-values:
R R work R free
0.224 0.221 0.276
Expression systems:
  • Escherichia coli
  • Not provided

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