› 7n1r

X-ray diffraction
2.03Å resolution

A novel and unique ATP hydrolysis to AMP by a human Hsp70 BiP

Released:
DOI: 10.2210/pdb7n1r/pdb
PDB entry 7n1r coloured by chain, front view.
PDB entry 7n1r coloured by chain, side view.
PDB entry 7n1r coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A novel and unique ATP hydrolysis to AMP by a human Hsp70 Binding immunoglobin protein (BiP).
Li H, Musayev FN, Yang J, Su J, Liu Q, Wang W, Fang X, Zhou L, Liu Q
Protein Sci 31 797-810 (2022)
PMID: 34941000

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-536318 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endoplasmic reticulum chaperone BiP Chains: A, B
Molecule details ›
Chains: A, B
Length: 606 amino acids
Theoretical weight: 67.24 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P11021 (Residues: 25-633; Coverage: 95%)
Gene names: GRP78, HSPA5
Sequence domains: Hsp70 protein

Ligands and Environments

6 bound ligands:
Ligand GOL 8 x GOL
Ligand SO4 6 x SO4
Ligand PO4 14 x PO4
Ligand MG 2 x MG
Ligand AMP 2 x AMP
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4C
Spacegroup: P1
Unit cell:
a: 75.988Å b: 76.388Å c: 79.917Å
α: 84.88° β: 62.49° γ: 63.14°
R-values:
R R work R free
0.177 0.174 0.223
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 citation in other articles

A new adenine nucleotide transporter located in the ER is essential for maintaining the growth of Toxoplasma gondii.
Li et al. (2022)
2 more

1 mention without citation

A novel and unique ATP hydrolysis to AMP by a human Hsp70 Binding immunoglobin protein (BiP).
Li et al. (2022)