› 7n36

X-ray diffraction
2Å resolution

Crystal structure of wild-type human gamma(S)-crystallin

Released:
DOI: 10.2210/pdb7n36/pdb
PDB entry 7n36 coloured by chain, front view.
PDB entry 7n36 coloured by chain, side view.
PDB entry 7n36 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Deamidation of the human eye lens protein γS-crystallin accelerates oxidative aging.
Norton-Baker B, Mehrabi P, Kwok AO, Roskamp KW, Rocha MA, Sprague-Piercy MA, von Stetten D, Miller RJD, Martin RW
Structure 30 763-776.e4 (2022)
PMID: 35338852

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149840 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Gamma-crystallin S Chains: A, B
Molecule details ›
Chains: A, B
Length: 178 amino acids
Theoretical weight: 20.96 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P22914 (Residues: 2-178; Coverage: 99%)
Gene names: CRYG8, CRYGS
Sequence domains: Beta/Gamma crystallin

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Spacegroup: P21
Unit cell:
a: 52.84Å b: 60.63Å c: 56.03Å
α: 90° β: 117.462° γ: 90°
R-values:
R R work R free
0.191 0.189 0.22
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Role of Oxidative Stress in Ocular Diseases: A Balancing Act.
Shu et al. (2023)
2 more

2 mentions without citation

Deamidation of the human eye lens protein γS-crystallin accelerates oxidative aging.
Norton-Baker et al. (2022)
1 more