› 7n38

X-ray diffraction
1.22Å resolution

Crystal structure of 5-site deamidated variant of human gamma(S)-crystallin

Released:
DOI: 10.2210/pdb7n38/pdb
PDB entry 7n38 coloured by chain, front view.
PDB entry 7n38 coloured by chain, side view.
PDB entry 7n38 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Deamidation of the human eye lens protein γS-crystallin accelerates oxidative aging.
Norton-Baker B, Mehrabi P, Kwok AO, Roskamp KW, Rocha MA, Sprague-Piercy MA, von Stetten D, Miller RJD, Martin RW
Structure 30 763-776.e4 (2022)
PMID: 35338852

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149840 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Gamma-crystallin S Chain: A
Molecule details ›
Chain: A
Length: 178 amino acids
Theoretical weight: 20.96 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P22914 (Residues: 2-178; Coverage: 99%)
Gene names: CRYG8, CRYGS
Sequence domains: Beta/Gamma crystallin

Ligands and Environments

2 bound ligands:
Ligand BTB 1 x BTB
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Spacegroup: P212121
Unit cell:
a: 29.315Å b: 62.694Å c: 79.805Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.148 0.146 0.179
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Role of Oxidative Stress in Ocular Diseases: A Balancing Act.
Shu et al. (2023)
2 more

1 mention without citation

Deamidation of the human eye lens protein γS-crystallin accelerates oxidative aging.
Norton-Baker et al. (2022)