› 7n3o

Electron Microscopy
3.8Å resolution

Cryo-EM structure of the Cas12k-sgRNA complex

Released:
DOI: 10.2210/pdb7n3o/pdb
PDB entry 7n3o coloured by chain, front view.
PDB entry 7n3o coloured by chain, side view.
PDB entry 7n3o coloured by chain, top view.
Source organism: Scytonema hofmannii
Primary publication:
Structural basis of target DNA recognition by CRISPR-Cas12k for RNA-guided DNA transposition.
Xiao R, Wang S, Han R, Li Z, Gabel C, Mukherjee IA, Chang L
Mol Cell 81 4457-4466.e5 (2021)
PMID: 34450043
Related structures: EMD-24142

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-168522 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Cas12k Chain: A
Molecule details ›
Chain: A
Length: 639 amino acids
Theoretical weight: 73.28 KDa
Source organism: Scytonema hofmannii
Expression system: Escherichia coli BL21(DE3)
Single guide RNA Chain: B
Molecule details ›
Chain: B
Length: 265 nucleotides
Theoretical weight: 85.26 KDa
Source organism: Scytonema hofmannii
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.8Å
Relevant EMDB volumes: EMD-24142
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

9 review citations

CRISPR-based genome editing through the lens of DNA repair.
Nambiar et al. (2022)
8 more

1 mention without citation

Structural basis of target DNA recognition by CRISPR-Cas12k for RNA-guided DNA transposition.
Xiao et al. (2021)