7n4k

X-ray diffraction
1.85Å resolution

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-100454 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
H-2 class I histocompatibility antigen, D-B alpha chain Chain: A
Molecule details ›
Chain: A
Length: 277 amino acids
Theoretical weight: 32.18 KDa
Source organism: Mus musculus
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P01899 (Residues: 25-301; Coverage: 82%)
Gene name: H2-D1
Sequence domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Polymerase acidic protein Chain: C
Molecule details ›
Chain: C
Length: 10 amino acids
Theoretical weight: 1.19 KDa
Source organism: Influenza A virus
Expression system: Not provided
UniProt:
  • Canonical: O89752 (Residues: 224-233; Coverage: 1%)
Gene name: PA
Ig-like domain-containing protein Chain: D
Molecule details ›
Chain: D
Length: 199 amino acids
Theoretical weight: 22.01 KDa
Source organisms: Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: K7N5N2 (Residues: 4-8, 115-204; Coverage: 46%)
  • Canonical: A0A075B6C4 (Residues: 25-107; Coverage: 92%)
Gene name: Trav21-dv12
Sequence domains:
Ig-like domain-containing protein Chain: E
Molecule details ›
Chain: E
Length: 240 amino acids
Theoretical weight: 27.58 KDa
Source organisms: Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: A0A0G2LB96 (Residues: 20-113; Coverage: 99%)
  • Canonical: K7N5M4 (Residues: 107-249; Coverage: 55%)
Gene names: B2M, HDCMA22P, Trbv28, Trbv29
Sequence domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P21
Unit cell:
a: 54.14Å b: 72.53Å c: 107.72Å
α: 90° β: 101.36° γ: 90°
R-values:
R R work R free
0.187 0.186 0.214
Expression systems:
  • Escherichia coli 'BL21-Gold(DE3)pLysS AG'
  • Not provided