› 7na7

Electron Microscopy
2.7Å resolution

Structures of human ghrelin receptor-Gi complexes with ghrelin and a synthetic agonist

Released:
DOI: 10.2210/pdb7na7/pdb
PDB entry 7na7 coloured by chain, front view.
PDB entry 7na7 coloured by chain, side view.
PDB entry 7na7 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of human ghrelin receptor signaling by ghrelin and the synthetic agonist ibutamoren
Liu H, Sun D, Myasnikov A, Damian M, Baneres JL, Sun J, Zhang C
Nat Commun 12 6410- (2021)
Related structures: EMD-24267

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-211371 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 354 amino acids
Theoretical weight: 40.43 KDa
Source organism: Homo sapiens
Expression system: Insect expression vector pBlueBacmsGCA1His
UniProt:
  • Canonical: P63096 (Residues: 1-354; Coverage: 100%)
Gene name: GNAI1
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 340 amino acids
Theoretical weight: 37.42 KDa
Source organism: Homo sapiens
Expression system: Insect expression vector pBlueBacmsGCA1His
UniProt:
  • Canonical: P62873 (Residues: 1-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Insect expression vector pBlueBacmsGCA1His
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Antibody fragment Chain: N
Molecule details ›
Chain: N
Length: 246 amino acids
Theoretical weight: 26.24 KDa
Source organism: Mus musculus
Expression system: Insect expression vector pBlueBacmsGCA1His
Growth hormone secretagogue receptor type 1 Chain: R
Molecule details ›
Chain: R
Length: 366 amino acids
Theoretical weight: 41.41 KDa
Source organism: Homo sapiens
Expression system: Insect expression vector pBlueBacmsGCA1His
UniProt:
  • Canonical: Q92847 (Residues: 1-366; Coverage: 100%)
Gene name: GHSR
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Ghrelin-27 Chain: L
Molecule details ›
Chain: L
Length: 12 amino acids
Theoretical weight: 1.47 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q9UBU3 (Residues: 24-35; Coverage: 13%)
Gene names: GHRL, MTLRP, UNQ524/PRO1066
Sequence domains: Motilin/ghrelin

Ligands and Environments

1 bound ligand:
Ligand CLR 2 x CLR
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.7Å
Relevant EMDB volumes: EMD-24267
Expression systems:
  • Insect expression vector pBlueBacmsGCA1His
  • Not provided

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