› 7nbp

Solution NMR

Solution structure of DNA duplex containing a 7,8-dihydro-8-oxo-1,N6-ethenoadenine base modification that induces exclusively A->T transversions in Escherichia coli

Released:
DOI: 10.2210/pdb7nbp/pdb
PDB entry 7nbp coloured by chain, ensemble of 10 models, front view.
PDB entry 7nbp coloured by chain, ensemble of 10 models, side view.
PDB entry 7nbp coloured by chain, ensemble of 10 models, top view.
Source organism: synthetic construct
Primary publication:
7,8-Dihydro-8-oxo-1,N6-ethenoadenine: an exclusively Hoogsteen-paired thymine mimic in DNA that induces A→T transversions in Escherichia coli.
Aralov AV, Gubina N, Cabrero C, Tsvetkov VB, Turaev AV, Fedeles BI, Croy RG, Isaakova EA, Melnik D, Dukova S, Ryazantsev DY, Khrulev AA, Varizhuk AM, González C, Zatsepin TS, Essigmann JM
OpenAccess logo Nucleic Acids Res 50 3056-3069 (2022)
PMID: 35234900

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-121688 (preferred)
Entry contents:
2 distinct DNA molecules
Macromolecules (2 distinct):
DNA (5'-D(*GP*TP*AP*CP*(EDA)P*CP*AP*TP*G)-3') Chain: A
Molecule details ›
Chain: A
Length: 9 nucleotides
Theoretical weight: 2.76 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(*CP*AP*TP*GP*AP*GP*TP*AP*C)-3') Chain: B
Molecule details ›
Chain: B
Length: 9 nucleotides
Theoretical weight: 2.74 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 37%
Refinement method: molecular dynamics
Expression system: Not provided

Quick links

Citations

1 review citation

Establishing Linkages Among DNA Damage, Mutagenesis, and Genetic Diseases.
Basu et al. (2022)
1 other article

1 mention without citation

7,8-Dihydro-8-oxo-1,N6-ethenoadenine: an exclusively Hoogsteen-paired thymine mimic in DNA that induces A→T transversions in Escherichia coli.
Aralov et al. (2022)