› 7nem

X-ray diffraction
1.35Å resolution

Hydrogenase-2 variant R479K - anaerobically oxidised form

Released:
DOI: 10.2210/pdb7nem/pdb
PDB entry 7nem coloured by chain, front view.
PDB entry 7nem coloured by chain, side view.
PDB entry 7nem coloured by chain, top view.
Source organism: Escherichia coli K-12
Entry author: Carr SB

Function and Biology Details

Reaction catalysed: 
H(2) + A = AH(2)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142265 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Hydrogenase-2 small chain Chains: S, T
Molecule details ›
Chains: S, T
Length: 298 amino acids
Theoretical weight: 32.37 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P69741 (Residues: 39-330; Coverage: 87%)
Gene names: JW2965, b2997, hybO, yghV
Sequence domains:
Hydrogenase-2 large chain Chains: L, M
Molecule details ›
Chains: L, M
Length: 567 amino acids
Theoretical weight: 62.53 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ACE0 (Residues: 1-567; Coverage: 100%)
Gene names: JW2962, b2994, hybC
Sequence domains: Nickel-dependent hydrogenase

Ligands and Environments

7 bound ligands:
Ligand SF4 4 x SF4
Ligand F3S 2 x F3S
Ligand FCO 2 x FCO
Ligand NI 2 x NI
Ligand MG 4 x MG
Ligand OXY 2 x OXY
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P212121
Unit cell:
a: 99.402Å b: 100.252Å c: 168.536Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.15 0.15 0.167
Expression system: Escherichia coli

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