› 7nf4

X-ray diffraction
1.69Å resolution

Structure of A. niger Fdc T395M R435P P438W variant (AnFdcII) in complex with prFMN

Released:
DOI: 10.2210/pdb7nf4/pdb
PDB entry 7nf4 coloured by chain, front view.
PDB entry 7nf4 coloured by chain, side view.
PDB entry 7nf4 coloured by chain, top view.
Source organism: Aspergillus niger CBS 513.88
Primary publication:
Directed evolution of prenylated FMN-dependent Fdc supports efficient in vivo isobutene production.
Saaret A, Villiers B, Stricher F, Anissimova M, Cadillon M, Spiess R, Hay S, Leys D
OpenAccess logo Nat Commun 12 5300 (2021)
PMID: 34489427

Function and Biology Details

Reaction catalysed: 
4-coumarate = 4-vinylphenol + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106926 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ferulic acid decarboxylase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 508 amino acids
Theoretical weight: 56.4 KDa
Source organism: Aspergillus niger CBS 513.88
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A2QHE5 (Residues: 1-500; Coverage: 100%)
Gene names: An03g06590, fdc1
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand MN 2 x MN
Ligand K 5 x K
Ligand BYN 2 x BYN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P212121
Unit cell:
a: 64.45Å b: 97.01Å c: 177.03Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.191 0.22
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

Toolbox for the structure-guided evolution of ferulic acid decarboxylase (FDC).
Duță et al. (2022)

1 mention without citation

Directed evolution of prenylated FMN-dependent Fdc supports efficient in vivo isobutene production.
Saaret et al. (2021)