› 7nhh

X-ray diffraction
2.1Å resolution

Crystal structure of the human METTL3-METTL14 complex with compound UOZ002

Released:
DOI: 10.2210/pdb7nhh/pdb
PDB entry 7nhh coloured by chain, front view.
PDB entry 7nhh coloured by chain, side view.
PDB entry 7nhh coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure-Based Design of Inhibitors of the m6A-RNA Writer Enzyme METTL3
Bedi RK, Huang D, Li Y, Caflisch A
Acs Bio Med Chem Au (2023)

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + adenine in mRNA = S-adenosyl-L-homocysteine + N(6)-methyladenine in mRNA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183176 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
N(6)-adenosine-methyltransferase catalytic subunit METTL3 Chain: A
Molecule details ›
Chain: A
Length: 246 amino acids
Theoretical weight: 28.14 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q86U44 (Residues: 354-580; Coverage: 39%)
Gene names: METTL3, MTA70
Sequence domains: MT-A70
N(6)-adenosine-methyltransferase non-catalytic subunit METTL14 Chain: B
Molecule details ›
Chain: B
Length: 290 amino acids
Theoretical weight: 33.62 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9HCE5 (Residues: 106-395; Coverage: 64%)
Gene names: KIAA1627, METTL14
Sequence domains: MT-A70

Ligands and Environments

3 bound ligands:
Ligand MG 4 x MG
Ligand UDK 1 x UDK
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P3221
Unit cell:
a: 64.05Å b: 64.05Å c: 226.02Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.209 0.207 0.25
Expression system: Spodoptera frugiperda

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