› 7nnp

Electron Microscopy
3.2Å resolution

Rb-loaded cryo-EM structure of the E1-ATP KdpFABC complex.

Released:
DOI: 10.2210/pdb7nnp/pdb
PDB entry 7nnp coloured by chain, front view.
PDB entry 7nnp coloured by chain, side view.
PDB entry 7nnp coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Deciphering ion transport and ATPase coupling in the intersubunit tunnel of KdpFABC.
Silberberg JM, Corey RA, Hielkema L, Stock C, Stansfeld PJ, Paulino C, Hänelt I
OpenAccess logo Nat Commun 12 5098 (2021)
PMID: 34429416
Related structures: EMD-12482

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + K(+)(Side 1) = ADP + phosphate + K(+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137301 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Potassium-transporting ATPase potassium-binding subunit Chain: A
Molecule details ›
Chain: A
Length: 557 amino acids
Theoretical weight: 59.28 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P03959 (Residues: 1-557; Coverage: 100%)
Gene names: JW0686, b0698, kdpA
Sequence domains: Potassium-transporting ATPase A subunit
Potassium-transporting ATPase KdpC subunit Chain: C
Molecule details ›
Chain: C
Length: 190 amino acids
Theoretical weight: 20.28 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P03961 (Residues: 1-190; Coverage: 100%)
Gene names: JW0684, b0696, kdpC
Sequence domains: K+-transporting ATPase, c chain
Potassium-transporting ATPase KdpF subunit Chain: D
Molecule details ›
Chain: D
Length: 27 amino acids
Theoretical weight: 2.85 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P36937 (Residues: 1-27; Coverage: 93%)
Gene names: JW0687, b4513, kdpF
Sequence domains: F subunit of K+-transporting ATPase (Potass_KdpF)
Potassium-transporting ATPase ATP-binding subunit Chain: B
Molecule details ›
Chain: B
Length: 682 amino acids
Theoretical weight: 72.25 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P03960 (Residues: 1-682; Coverage: 100%)
Gene names: JW0685, b0697, kdpB
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand RB 8 x RB
Ligand CDL 2 x CDL
Ligand ACP 1 x ACP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-12482
Expression system: Escherichia coli

Quick links

Citations

1 review citation

P-type ATPases: Many more enigmas left to solve.
Palmgren M. (2023)
3 other articles

1 mention without citation

Inhibited KdpFABC transitions into an E1 off-cycle state.
Silberberg et al. (2022)