› 7nnq

X-ray diffraction
1.73Å resolution

Crystal structure of Mycobacterium tuberculosis ArgC in complex with nicotinamide adenine dinucleotide phosphate (NADP+)

Released:
DOI: 10.2210/pdb7nnq/pdb
PDB entry 7nnq coloured by chain, front view.
PDB entry 7nnq coloured by chain, side view.
PDB entry 7nnq coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
A fragment-based approach to assess the ligandability of ArgB, ArgC, ArgD and ArgF in the L-arginine biosynthetic pathway of Mycobacterium tuberculosis
Gupta P, Thomas SE, Zaidan SA, Pasillas MA, Cory-Wright J, Sebastian-Perez V, Burgess A, Cattermole E, Meghir C, Abell C, Coyne AG, Jacobs WR, Blundell TL, Tiwari S, Mendes V
Comput Struct Biotechnol J 19 3491-3506 (2021)

Function and Biology Details

Reaction catalysed: 
N-acetyl-L-glutamate 5-semialdehyde + NADP(+) + phosphate = N-acetyl-L-glutamyl 5-phosphate + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-162037 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-acetyl-gamma-glutamyl-phosphate reductase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 344 amino acids
Theoretical weight: 35.4 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WPZ9 (Residues: 9-352; Coverage: 98%)
Gene names: MTCY06H11.17, Rv1652, argC
Sequence domains:

Ligands and Environments


Cofactor: Ligand NAP 4 x NAP
1 bound ligand:
Ligand BTB 3 x BTB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P21
Unit cell:
a: 69.05Å b: 133.83Å c: 83.05Å
α: 90° β: 109.39° γ: 90°
R-values:
R R work R free
0.183 0.182 0.208
Expression system: Escherichia coli BL21(DE3)

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