› 7no2

Electron Microscopy
4.3Å resolution

Structure of the mature RSV CA lattice: hexamer derived from tubes (C2-symmetric)

Released:
DOI: 10.2210/pdb7no2/pdb
PDB entry 7no2 coloured by chain, front view.
PDB entry 7no2 coloured by chain, side view.
PDB entry 7no2 coloured by chain, top view.
Source organism: Rous sarcoma virus - Prague C
Primary publication:
Structure of the mature Rous sarcoma virus lattice reveals a role for IP6 in the formation of the capsid hexamer.
Obr M, Ricana CL, Nikulin N, Feathers JR, Klanschnig M, Thader A, Johnson MC, Vogt VM, Schur FKM, Dick RA
OpenAccess logo Nat Commun 12 3226 (2021)
PMID: 34050170
Related structures: EMD-12487

Function and Biology Details

Reactions catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136822 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Capsid protein p27, alternate cleaved 1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 229 amino acids
Theoretical weight: 24.77 KDa
Source organism: Rous sarcoma virus - Prague C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03354 (Residues: 240-468; Coverage: 14%)
Gene name: gag-pol
Sequence domains: gag protein p24 N-terminal domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.3Å
Relevant EMDB volumes: EMD-12487
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Structural Analysis of Retrovirus Assembly and Maturation.
Krebs et al. (2021)
2 more

1 mention without citation

A Structural Perspective of the Role of IP6 in Immature and Mature Retroviral Assembly.
Obr et al. (2021)