› 7nt0

X-ray diffraction
1.8Å resolution

Drosophila PGRP-LB Y78F mutant in complex with tracheal cytotoxin (TCT)

Released:
DOI: 10.2210/pdb7nt0/pdb
PDB entry 7nt0 coloured by chain, front view.
PDB entry 7nt0 coloured by chain, side view.
PDB entry 7nt0 coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
PGRP-LB: An Inside View into the Mechanism of the Amidase Reaction.
Orlans J, Vincent-Monegat C, Rahioui I, Sivignon C, Butryn A, Soulère L, Zaidman-Remy A, Orville AM, Heddi A, Aller P, Da Silva P
OpenAccess logo Int J Mol Sci 22 (2021)
PMID: 34066955

Function and Biology Details

Reaction catalysed: 
Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184830 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptidoglycan-recognition protein LB Chains: AAA, BBB
Molecule details ›
Chains: AAA, BBB
Length: 217 amino acids
Theoretical weight: 23.77 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8INK6 (Residues: 18-232; Coverage: 99%)
Gene names: CG14704, PGRP-LB
Sequence domains: N-acetylmuramoyl-L-alanine amidase

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand MLD 2 x MLD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P1
Unit cell:
a: 38.587Å b: 52.167Å c: 55.579Å
α: 92.113° β: 105.123° γ: 111.513°
R-values:
R R work R free
0.146 0.145 0.18
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 citation in other articles

Pharmacological Effect of Caffeine on Drosophila melanogaster: A Proof-of-Concept in vivo Study for Nootropic Investigation.
Asbah et al. (2021)
2 more

2 mentions without citation

Structural insights into the SARS-CoV-2 Omicron RBD-ACE2 interaction.
Lan et al. (2022)
1 more