› 7nuh

X-ray diffraction
2.2Å resolution

Influenza A/California/07/2009(H1N1) endonuclease with I38T mutation in complex with orientin

Released:
DOI: 10.2210/pdb7nuh/pdb
PDB entry 7nuh coloured by chain, front view.
PDB entry 7nuh coloured by chain, side view.
PDB entry 7nuh coloured by chain, top view.
Source organism: Influenza A virus (A/California/07/2009(H1N1))
Primary publication:
Synthesis and In Vitro Evaluation of C-7 and C-8 Luteolin Derivatives as Influenza Endonuclease Inhibitors.
Reiberger R, Radilová K, Kráľ M, Zima V, Majer P, Brynda J, Dračínský M, Konvalinka J, Kožíšek M, Machara A
OpenAccess logo Int J Mol Sci 22 (2021)
PMID: 34299354

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111254 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polymerase acidic protein Chain: A
Molecule details ›
Chain: A
Length: 180 amino acids
Theoretical weight: 21.2 KDa
Source organism: Influenza A virus (A/California/07/2009(H1N1))
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C3W5X6 (Residues: 1-50, 73-196; Coverage: 24%)
Gene name: PA

Ligands and Environments

5 bound ligands:
Ligand MN 2 x MN
Ligand MG 1 x MG
Ligand PEG 2 x PEG
Ligand EDO 4 x EDO
Ligand USE 1 x USE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P6422
Unit cell:
a: 73.967Å b: 73.967Å c: 127.575Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.196 0.193 0.264
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Evolution of Influenza Viruses-Drug Resistance, Treatment Options, and Prospects.
Smyk et al. (2022)
2 more

1 mention without citation

Synthesis and In Vitro Evaluation of C-7 and C-8 Luteolin Derivatives as Influenza Endonuclease Inhibitors.
Reiberger et al. (2021)