› 7nuj

X-ray diffraction
1.9Å resolution

Crystal structure of holo-SwHPA-Mg (hydroxy ketone aldolase) from Sphingomonas wittichii RW1

Released:
DOI: 10.2210/pdb7nuj/pdb
Model geometry
Fit model/data
PDB entry 7nuj coloured by chain, front view.
PDB entry 7nuj coloured by chain, side view.
PDB entry 7nuj coloured by chain, top view.
Source organism: Rhizorhabdus wittichii RW1
Primary publication:
Substrate Induced Movement of the Metal Cofactor between Active and Resting State.
Marsden SR, Wijma HJ, Mohr MKF, Justo I, Hagedoorn PL, Laustsen J, Jeffries CM, Svergun D, Mestrom L, McMillan DGG, Bento I, Hanefeld U
OpenAccess logo Angew Chem Int Ed Engl (2022)
PMID: 36214476

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-290444 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HpcH/HpaI aldolase/citrate lyase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 252 amino acids
Theoretical weight: 26.78 KDa
Source organism: Rhizorhabdus wittichii RW1
Expression system: Escherichia coli
UniProt:
  • Canonical: A0AAT8XT73 (Residues: 3-252; Coverage: 99%)
Gene name: Swit_5035

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Spacegroup: R3
Unit cell:
a: 71.102Å b: 71.102Å c: 223.196Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.167 0.165 0.204
Expression system: Escherichia coli

Quick links

Citations

1 mention without citation

Substrate Induced Movement of the Metal Cofactor between Active and Resting State.
Marsden et al. (2022)