7nv0

Electron Microscopy
3.4Å resolution

Human Pol Kappa holoenzyme with wt PCNA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-121702 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
DNA polymerase kappa Chain: A
Molecule details ›
Chain: A
Length: 870 amino acids
Theoretical weight: 98.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UBT6 (Residues: 1-870; Coverage: 100%)
Gene names: DINB1, POLK
Sequence domains:
Proliferating cell nuclear antigen Chains: B, C, D
Molecule details ›
Chains: B, C, D
Length: 264 amino acids
Theoretical weight: 29.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P12004 (Residues: 1-261; Coverage: 100%)
Gene name: PCNA
Sequence domains:
DNA Primer Chain: P
Molecule details ›
Chain: P
Length: 25 nucleotides
Theoretical weight: 7.67 KDa
DNA Template Chain: T
Molecule details ›
Chain: T
Length: 38 nucleotides
Theoretical weight: 11.68 KDa

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
Resolution: 3.4Å
Relevant EMDB volumes: EMD-12601
Expression system: Escherichia coli BL21(DE3)