› 7nvm

Electron Microscopy
3.1Å resolution

Human TRiC complex in closed state with nanobody Nb18, actin and PhLP2A bound

Released:
DOI: 10.2210/pdb7nvm/pdb
PDB entry 7nvm coloured by chain, front view.
PDB entry 7nvm coloured by chain, side view.
PDB entry 7nvm coloured by chain, top view.
Source organisms:
Primary publication:
Snapshots of actin and tubulin folding inside the TRiC chaperonin.
Kelly JJ, Tranter D, Pardon E, Chi G, Kramer H, Happonen L, Knee KM, Janz JM, Steyaert J, Bulawa C, Paavilainen VO, Huiskonen JT, Yue WW
OpenAccess logo Nat Struct Mol Biol 29 420-429 (2022)
PMID: 35449234
Related structures: EMD-12606

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero icosamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-276162 (preferred)
Entry contents:
11 distinct polypeptide molecules
Macromolecules (11 distinct):
T-complex protein 1 subunit alpha Chains: A, a
Molecule details ›
Chains: A, a
Length: 556 amino acids
Theoretical weight: 60.42 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P17987 (Residues: 1-556; Coverage: 100%)
Gene names: CCT1, CCTA, TCP1
Sequence domains: TCP-1/cpn60 chaperonin family
T-complex protein 1 subunit beta Chains: B, b
Molecule details ›
Chains: B, b
Length: 535 amino acids
Theoretical weight: 57.57 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P78371 (Residues: 1-535; Coverage: 100%)
Gene names: 99D8.1, CCT2, CCTB
Sequence domains: TCP-1/cpn60 chaperonin family
T-complex protein 1 subunit delta Chains: D, d
Molecule details ›
Chains: D, d
Length: 539 amino acids
Theoretical weight: 58 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P50991 (Residues: 1-539; Coverage: 100%)
Gene names: CCT4, CCTD, SRB
Sequence domains: TCP-1/cpn60 chaperonin family
T-complex protein 1 subunit epsilon Chains: E, e
Molecule details ›
Chains: E, e
Length: 541 amino acids
Theoretical weight: 59.75 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P48643 (Residues: 1-541; Coverage: 100%)
Gene names: CCT5, CCTE, KIAA0098
Sequence domains: TCP-1/cpn60 chaperonin family
T-complex protein 1 subunit gamma Chains: G, g
Molecule details ›
Chains: G, g
Length: 545 amino acids
Theoretical weight: 60.61 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P49368 (Residues: 1-545; Coverage: 100%)
Gene names: CCT3, CCTG, TRIC5
Sequence domains: TCP-1/cpn60 chaperonin family
T-complex protein 1 subunit eta Chains: H, h
Molecule details ›
Chains: H, h
Length: 543 amino acids
Theoretical weight: 59.44 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q99832 (Residues: 1-543; Coverage: 100%)
Gene names: CCT7, CCTH, NIP7-1
Sequence domains: TCP-1/cpn60 chaperonin family
Nanobody Nb18 Chains: N, n
Molecule details ›
Chains: N, n
Length: 129 amino acids
Theoretical weight: 14.41 KDa
Source organism: Lama glama
Expression system: Escherichia coli
T-complex protein 1 subunit theta Chains: Q, q
Molecule details ›
Chains: Q, q
Length: 548 amino acids
Theoretical weight: 59.69 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P50990 (Residues: 1-548; Coverage: 100%)
Gene names: C21orf112, CCT8, CCTQ, KIAA0002
Sequence domains: TCP-1/cpn60 chaperonin family
T-complex protein 1 subunit zeta Chains: Z, z
Molecule details ›
Chains: Z, z
Length: 531 amino acids
Theoretical weight: 58.11 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P40227 (Residues: 1-531; Coverage: 100%)
Gene names: CCT6, CCT6A, CCTZ
Sequence domains: TCP-1/cpn60 chaperonin family
Actin, cytoplasmic 2 Chain: K
Molecule details ›
Chain: K
Length: 375 amino acids
Theoretical weight: 41.84 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P63261 (Residues: 1-375; Coverage: 100%)
Gene names: ACTG, ACTG1
Sequence domains: Actin
Phosducin-like protein 3 Chain: P
Molecule details ›
Chain: P
Length: 239 amino acids
Theoretical weight: 27.65 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9H2J4 (Residues: 1-239; Coverage: 100%)
Gene names: PDCL3, PhLP2A, VIAF1
Sequence domains: Phosducin

Ligands and Environments

3 bound ligands:
Ligand ADP 16 x ADP
Ligand MG 16 x MG
Ligand AF3 16 x AF3
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.1Å
Relevant EMDB volumes: EMD-12606
Expression systems:
  • Homo sapiens
  • Escherichia coli

Quick links

Citations

3 review citations

Regulation of Epithelial and Endothelial Barriers by Molecular Chaperones.
Lechuga et al. (2024)
2 more

6 mentions without citation

Mechanistic insights into protein folding by the eukaryotic chaperonin complex CCT.
Smith et al. (2022)
5 more