› 7nvw

Electron Microscopy
4.3Å resolution

TFIIH in a pre-translocated state (without ADP-BeF3)

Released:
DOI: 10.2210/pdb7nvw/pdb
PDB entry 7nvw coloured by chain, front view.
PDB entry 7nvw coloured by chain, side view.
PDB entry 7nvw coloured by chain, top view.
Source organisms:
Primary publication:
Structures of mammalian RNA polymerase II pre-initiation complexes.
Aibara S, Schilbach S, Cramer P
Nature 594 124-128 (2021)
PMID: 33902107
Related structures: EMD-12615

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tridecamer (preferred)
PDBe Complex ID:
PDB-CPX-121705 (preferred)
Entry contents:
11 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (13 distinct):
General transcription and DNA repair factor IIH helicase subunit XPD Chain: 0
Molecule details ›
Chain: 0
Length: 760 amino acids
Theoretical weight: 87.02 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
General transcription factor IIH subunit 1 Chain: 1
Molecule details ›
Chain: 1
Length: 548 amino acids
Theoretical weight: 62.12 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
General transcription factor IIH subunit 4 Chain: 2
CDK-activating kinase assembly factor MAT1 Chain: 3
General transcription factor IIH subunit 3 Chain: 4
General transcription factor IIH subunit 5 Chain: 5
General transcription factor IIH subunit 2 Chain: 6
General transcription and DNA repair factor IIH helicase/translocase subunit XPB Chain: 7
General transcription factor IIE subunit 1 Chain: W
Unassigned Peptide, likely XPB Chain: Y
Unassigned Peptide, likely TFIIE-Beta Chain: Z
Non-template DNA Chain: N
Template DNA Chain: T

Ligands and Environments

2 bound ligands:
Ligand SF4 1 x SF4
Ligand ZN 7 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.3Å
Relevant EMDB volumes: EMD-12615
Expression systems:
  • Trichoplusia ni
  • Escherichia coli
  • Not provided

Quick links

Citations

8 review citations

The Mediator complex as a master regulator of transcription by RNA polymerase II.
Richter et al. (2022)
7 more

4 mentions without citation

Assembly of RNA polymerase II transcription initiation complexes.
Farnung et al. (2022)
3 more