7nwu

X-ray diffraction
2.6Å resolution

Co-crystal structure of UPF3B-RRM-NOPS-L with UPF2-MIF4GIII

Released:
Model geometry
Fit model/data

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-189860 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Regulator of nonsense transcripts 3B Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 122 amino acids
Theoretical weight: 14.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9BZI7 (Residues: 49-170; Coverage: 25%)
Gene names: RENT3B, UPF3B, UPF3X
Sequence domains: Smg-4/UPF3 family
Regulator of nonsense transcripts 2 Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 251 amino acids
Theoretical weight: 29.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9HAU5 (Residues: 767-1017; Coverage: 20%)
Gene names: KIAA1408, RENT2, UPF2
Sequence domains: MIF4G domain

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P4122
Unit cell:
a: 130.593Å b: 130.593Å c: 267.33Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.217 0.256
Expression system: Escherichia coli BL21(DE3)