› 7nyb

X-ray diffraction
2.5Å resolution

TrmB complex with SAM

Released:
DOI: 10.2210/pdb7nyb/pdb
PDB entry 7nyb coloured by chain, front view.
PDB entry 7nyb coloured by chain, side view.
PDB entry 7nyb coloured by chain, top view.
Source organism: Bacillus subtilis subsp. subtilis str. 168
Primary publication:
Structural model of the M7G46 Methyltransferase TrmB in complex with tRNA.
Blersch KF, Burchert JP, August SC, Welp L, Neumann P, Köster S, Urlaub H, Ficner R
RNA Biol 18 2466-2479 (2021)
PMID: 34006170

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128550 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA (guanine-N(7)-)-methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 213 amino acids
Theoretical weight: 24.54 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli
UniProt:
  • Canonical: O34522 (Residues: 1-213; Coverage: 100%)
Gene names: BSU29900, trmB, ytmQ
Sequence domains: Putative methyltransferase

Ligands and Environments


Cofactor: Ligand SAM 2 x SAM
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Spacegroup: R3
Unit cell:
a: 178.19Å b: 178.19Å c: 37.01Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.211 0.209 0.254
Expression system: Escherichia coli

Quick links

Citations

1 review citation

The role of RNA modification in hepatocellular carcinoma.
Feng et al. (2022)
5 other articles

1 mention without citation

Structural model of the M7G46 Methyltransferase TrmB in complex with tRNA.
Blersch et al. (2021)