› 7nyw

Electron Microscopy
3.1Å resolution

Cryo-EM structure of the MukBEF-MatP-DNA head module

Released:
DOI: 10.2210/pdb7nyw/pdb
PDB entry 7nyw coloured by chain, front view.
PDB entry 7nyw coloured by chain, side view.
PDB entry 7nyw coloured by chain, top view.
Source organisms:
Primary publication:
Cryo-EM structure of MukBEF reveals DNA loop entrapment at chromosomal unloading sites.
Bürmann F, Funke LFH, Chin JW, Löwe J
OpenAccess logo Mol Cell 81 4891-4906.e8 (2021)
PMID: 34739874
Related structures: EMD-12656

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetradecamer (preferred)
PDBe Complex ID:
PDB-CPX-101299 (preferred)
Entry contents:
5 distinct polypeptide molecules
3 distinct DNA molecules
Macromolecules (8 distinct):
Chromosome partition protein MukB Chains: A, B
Molecule details ›
Chains: A, B
Length: 1482 amino acids
Theoretical weight: 170.24 KDa
Source organism: Photorhabdus thracensis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0F7LRY2 (Residues: 1-1482; Coverage: 100%)
Gene names: VY86_15870, mukB
Sequence domains:
Chromosome partition protein MukF Chains: C, D
Molecule details ›
Chains: C, D
Length: 440 amino acids
Theoretical weight: 50.19 KDa
Source organism: Photorhabdus thracensis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0F7LMQ4 (Residues: 1-440; Coverage: 100%)
Gene names: VY86_15860, mukF
Sequence domains:
Chromosome partition protein MukE Chains: E, F
Acyl carrier protein Chains: G, H
Macrodomain Ter protein Chains: I, J
matS2 DNA 80 b, oligo FBA769 Chain: K
matS2 DNA 80 b, oligo FBA770 Chain: L
DNA 80 b Chains: M, N

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand ATP 2 x ATP
Ligand PNS 2 x PNS
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.1Å
Relevant EMDB volumes: EMD-12656
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

SMC complexes: Lifting the lid on loop extrusion.
Higashi et al. (2022)
3 more

2 mentions without citation

Cryo-EM structure of MukBEF reveals DNA loop entrapment at chromosomal unloading sites.
Bürmann et al. (2021)
1 more

Experimental raw data

Links to raw experimental data available for this entry are listed below

Electron microscopy data related to PDB entry 7nyw found at the Electron Microscopy Public Image Archive (EMPIAR)

Data DOI: 10.6019/EMPIAR-10755
Datasets: 5
Total size: 8500Gb
EMPIAR thumbnail