› 7o41

Electron Microscopy
7.6Å resolution

Hexameric composite model of the Inner Membrane Complex (IMC) with the Arches from the fully-assembled R388 type IV secretion system determined by cryo-EM.

Released:
DOI: 10.2210/pdb7o41/pdb
PDB entry 7o41 coloured by chain, front view.
PDB entry 7o41 coloured by chain, side view.
PDB entry 7o41 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Cryo-EM structure of a type IV secretion system.
Macé K, Vadakkepat AK, Redzej A, Lukoyanova N, Oomen C, Braun N, Ukleja M, Lu F, Costa TRD, Orlova EV, Baker D, Cong Q, Waksman G
OpenAccess logo Nature 607 191-196 (2022)
PMID: 35732732
Related structures: EMD-12715

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 36-mer (preferred)
PDBe Complex ID:
PDB-CPX-129318 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
TrwM protein Chain: C
Molecule details ›
Chain: C
Length: 104 amino acids
Theoretical weight: 12.29 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: O50329 (Residues: 1-104; Coverage: 100%)
Gene name: trwM
Sequence domains: Type IV secretory pathway, VirB3-like protein
Type IV secretion system protein virB4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 823 amino acids
Theoretical weight: 93.77 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: O50330 (Residues: 1-823; Coverage: 100%)
Gene name: trwK
Sequence domains:
Bacterial virulence protein VirB8 domain-containing protein Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 231 amino acids
Theoretical weight: 25.8 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: O50335 (Residues: 1-231; Coverage: 100%)
Gene name: trwG
Sequence domains: VirB8 protein

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 7.6Å
Relevant EMDB volumes: EMD-12715
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Structural and functional diversity of type IV secretion systems.
Costa et al. (2024)
8 more