› 7o7c

X-ray diffraction
1.55Å resolution

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 250 amino acids
Theoretical weight: 28.5 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42212 (Residues: 2-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
1 modified residue:
Ligand PIA 1 x PIA

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 51.16Å b: 60.29Å c: 66.61Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.164 0.196
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

Serial Femtosecond Crystallography Reveals that Photoactivation in a Fluorescent Protein Proceeds via the Hula Twist Mechanism.
Fadini et al. (2023)

1 mention without citation

Rational Control of Off-State Heterogeneity in a Photoswitchable Fluorescent Protein Provides Switching Contrast Enhancement.
Adam et al. (2022)