› 7o8j

X-ray diffraction
1.8Å resolution

NmHR light state structure at 20 us after photoexcitation determined by serial femtosecond crystallography (with extrapolated, dark and light dataset)

Released:
DOI: 10.2210/pdb7o8j/pdb
PDB entry 7o8j coloured by chain, front view.
PDB entry 7o8j coloured by chain, side view.
PDB entry 7o8j coloured by chain, top view.
Source organism: Nonlabens marinus S1-08
Primary publication:
Dynamics and mechanism of a light-driven chloride pump.
Mous S, Gotthard G, Ehrenberg D, Sen S, Weinert T, Johnson PJM, James D, Nass K, Furrer A, Kekilli D, Ma P, Brünle S, Casadei CM, Martiel I, Dworkowski F, Gashi D, Skopintsev P, Wranik M, Knopp G, Panepucci E, Panneels V, Cirelli C, Ozerov D, Schertler GFX, Wang M, Milne C, Standfuss J, Schapiro I, Heberle J, Nogly P
Science 375 845-851 (2022)
PMID: 35113649

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197820 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chloride pumping rhodopsin Chain: A
Molecule details ›
Chain: A
Length: 296 amino acids
Theoretical weight: 32.91 KDa
Source organism: Nonlabens marinus S1-08
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: W8VZW3 (Residues: 1-272; Coverage: 100%)
Gene names: ClR, NMS_1267
Sequence domains: Bacteriorhodopsin-like protein

Ligands and Environments

4 bound ligands:
Ligand RET 1 x RET
Ligand CL 2 x CL
Ligand OLC 3 x OLC
Ligand OLA 5 x OLA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SwissFEL ARAMIS BEAMLINE ESA
Spacegroup: C2
Unit cell:
a: 104.48Å b: 51.18Å c: 78.33Å
α: 90° β: 131.78° γ: 90°
R-values:
R R work R free
0.287 0.284 0.327
Expression system: Escherichia coli BL21(DE3)

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Citations

5 review citations

Convergent evolution of animal and microbial rhodopsins.
Kojima et al. (2023)
4 more