› 7oat

X-ray diffraction
3Å resolution

Structural basis for targeted p97 remodelling by ASPL as prerequisite for p97 trimethylation by METTL21D

Released:
DOI: 10.2210/pdb7oat/pdb
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PDB entry 7oat coloured by chain, front view.
PDB entry 7oat coloured by chain, side view.
PDB entry 7oat coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural remodeling of AAA+ ATPase p97 by adaptor protein ASPL facilitates posttranslational methylation by METTL21D.
Petrović S, Roske Y, Rami B, Phan MHQ, Panáková D, Heinemann U
OpenAccess logo Proc Natl Acad Sci U S A 120 e2208941120 (2023)
PMID: 36656859

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-157175 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Tether containing UBX domain for GLUT4 Chain: A
Molecule details ›
Chain: A
Length: 190 amino acids
Theoretical weight: 21.61 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9BZE9 (Residues: 313-500; Coverage: 34%)
Gene names: ASPL, ASPSCR1, RCC17, TUG, UBXD9, UBXN9
Sequence domains: UBX domain
Transitional endoplasmic reticulum ATPase Chain: B
Molecule details ›
Chain: B
Length: 481 amino acids
Theoretical weight: 53.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P55072 (Residues: 2-480; Coverage: 59%)
Gene names: HEL-220, HEL-S-70, VCP
Sequence domains:
Protein N-lysine methyltransferase METTL21D Chain: C

Ligands and Environments

4 bound ligands:
Ligand EDO 4 x EDO
Ligand ATP 1 x ATP
Ligand MG 1 x MG
Ligand SAH 1 x SAH
1 modified residue:
Ligand M3L 1 x M3L

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P21
Unit cell:
a: 54.258Å b: 69.622Å c: 140.175Å
α: 90° β: 94.41° γ: 90°
R-values:
R R work R free
0.228 0.225 0.27
Expression system: Escherichia coli

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Citations

1 review citation

Structural insights of the p97/VCP AAA+ ATPase: How adapter interactions coordinate diverse cellular functionality.
Braxton et al. (2023)
3 other articles

1 mention without citation

Structural remodeling of AAA+ ATPase p97 by adaptor protein ASPL facilitates posttranslational methylation by METTL21D.
Petrović et al. (2023)

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