› 7oba

Electron Microscopy
3.1Å resolution

Cryo-EM structure of human RNA Polymerase I in complex with RRN3

Released:
DOI: 10.2210/pdb7oba/pdb
PDB entry 7oba coloured by chain, front view.
PDB entry 7oba coloured by chain, side view.
PDB entry 7oba coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Cryo-EM structures of human RNA polymerase I.
Misiaszek AD, Girbig M, Grötsch H, Baudin F, Murciano B, Lafita A, Müller CW
Nat Struct Mol Biol 28 997-1008 (2021)
PMID: 34887565
Related structures: EMD-12796

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetradecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127334 (preferred)
Entry contents:
14 distinct polypeptide molecules
Macromolecules (14 distinct):
DNA-directed RNA polymerase I subunit RPA1 Chain: A
Molecule details ›
Chain: A
Length: 1720 amino acids
Theoretical weight: 195.07 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: O95602 (Residues: 1-1720; Coverage: 100%)
Gene name: POLR1A
Sequence domains:
DNA-directed RNA polymerase I subunit RPA2 Chain: B
Molecule details ›
Chain: B
Length: 1135 amino acids
Theoretical weight: 128.38 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9H9Y6 (Residues: 1-1135; Coverage: 100%)
Gene name: POLR1B
Sequence domains:
DNA-directed RNA polymerases I and III subunit RPAC1 Chain: C
Molecule details ›
Chain: C
Length: 346 amino acids
Theoretical weight: 39.3 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: O15160 (Residues: 1-346; Coverage: 100%)
Gene names: POLR1C, POLR1E
Sequence domains:
DNA-directed RNA polymerases I, II, and III subunit RPABC1 Chain: E
Molecule details ›
Chain: E
Length: 210 amino acids
Theoretical weight: 24.64 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P19388 (Residues: 1-210; Coverage: 100%)
Gene name: POLR2E
Sequence domains:
DNA-directed RNA polymerases I, II, and III subunit RPABC2 Chain: F
Molecule details ›
Chain: F
Length: 127 amino acids
Theoretical weight: 14.49 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P61218 (Residues: 1-127; Coverage: 100%)
Gene names: POLR2F, POLRF
Sequence domains: RNA polymerase Rpb6
DNA-directed RNA polymerase I subunit RPA43 Chain: G
Molecule details ›
Chain: G
Length: 338 amino acids
Theoretical weight: 37.49 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q3B726 (Residues: 1-338; Coverage: 100%)
Gene names: POLR1F, TWISTNB
Sequence domains:
DNA-directed RNA polymerases I, II, and III subunit RPABC3 Chain: H
Molecule details ›
Chain: H
Length: 150 amino acids
Theoretical weight: 17.16 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P52434 (Residues: 1-150; Coverage: 100%)
Gene name: POLR2H
Sequence domains: RNA polymerase Rpb8
DNA-directed RNA polymerase I subunit RPA12 Chain: I
Molecule details ›
Chain: I
Length: 126 amino acids
Theoretical weight: 13.92 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9P1U0 (Residues: 1-126; Coverage: 100%)
Gene names: POLR1H, RPA12, ZNRD1
Sequence domains: Transcription factor S-II (TFIIS)
DNA-directed RNA polymerases I, II, and III subunit RPABC5 Chain: J
Molecule details ›
Chain: J
Length: 67 amino acids
Theoretical weight: 7.66 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62875 (Residues: 1-67; Coverage: 100%)
Gene name: POLR2L
Sequence domains: RNA polymerases N / 8 kDa subunit
DNA-directed RNA polymerases I and III subunit RPAC2 Chain: K
Molecule details ›
Chain: K
Length: 133 amino acids
Theoretical weight: 15.26 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P0DPB6 (Residues: 1-133; Coverage: 100%)
Gene name: POLR1D
Sequence domains: RNA polymerase Rpb3/Rpb11 dimerisation domain
DNA-directed RNA polymerases I, II, and III subunit RPABC4 Chain: L
Molecule details ›
Chain: L
Length: 58 amino acids
Theoretical weight: 7.02 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P53803 (Residues: 1-58; Coverage: 100%)
Gene name: POLR2K
Sequence domains: DNA directed RNA polymerase, 7 kDa subunit
DNA-directed RNA polymerase I subunit RPA49 Chain: M
Molecule details ›
Chain: M
Length: 419 amino acids
Theoretical weight: 47.33 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9GZS1 (Residues: 1-419; Coverage: 100%)
Gene names: PAF53, POLR1E, PRAF1
Sequence domains: A49-like RNA polymerase I associated factor
DNA-directed RNA polymerase I subunit RPA34 Chain: N
Molecule details ›
Chain: N
Length: 510 amino acids
Theoretical weight: 55.07 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: O15446 (Residues: 1-510; Coverage: 100%)
  • Best match: O15446-2 (Residues: 1-512)
Gene names: ASE1, CAST, CD3EAP, PAF49, POLR1G
Sequence domains: DNA-directed RNA polymerase I subunit RPA34.5
RNA polymerase I-specific transcription initiation factor RRN3 Chain: O
Molecule details ›
Chain: O
Length: 651 amino acids
Theoretical weight: 74.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NYV6 (Residues: 1-651; Coverage: 100%)
Gene names: RRN3, TIFIA
Sequence domains: RNA polymerase I specific transcription initiation factor RRN3

Ligands and Environments

1 bound ligand:
Ligand ZN 7 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.1Å
Relevant EMDB volumes: EMD-12796
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Targeting Ribosome Biogenesis in Cancer: Lessons Learned and Way Forward.
Zisi et al. (2022)
6 more

4 mentions without citation

Ribosome biogenesis factors-from names to functions.
Dörner et al. (2023)
3 more