› 7obh

X-ray diffraction
2Å resolution

Crystal structure of 14-3-3 sigma in complex with NPM1 phosphopeptide and stabilizer Fusicoccin-A

Released:
DOI: 10.2210/pdb7obh/pdb
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PDB entry 7obh coloured by chain, front view.
PDB entry 7obh coloured by chain, side view.
PDB entry 7obh coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
IFNα primes cancer cells for Fusicoccin-induced cell death via 14-3-3 PPI stabilization.
Andlovic B, Heilmann G, Ninck S, Andrei SA, Centorrino F, Higuchi Y, Kato N, Brunsveld L, Arkin M, Menninger S, Choidas A, Wolf A, Klebl B, Kaschani F, Kaiser M, Eickhoff J, Ottmann C
Cell Chem Biol (2023)
PMID: 37130519

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139204 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3 protein sigma Chain: A
Molecule details ›
Chain: A
Length: 253 amino acids
Theoretical weight: 28.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P31947 (Residues: 1-248; Coverage: 100%)
Gene names: HME1, SFN
Sequence domains: 14-3-3 protein
Nucleophosmin Chain: B
Molecule details ›
Chain: B
Length: 11 amino acids
Theoretical weight: 1.55 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P06748 (Residues: 284-294; Coverage: 4%)
Gene names: NPM, NPM1

Ligands and Environments

2 bound ligands:
Ligand FSC 1 x FSC
Ligand CL 1 x CL
2 modified residues:
Ligand CSO 1 x CSO
Ligand SEP 1 x SEP

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: RIGAKU MICROMAX-003
Spacegroup: C2221
Unit cell:
a: 82.475Å b: 112.195Å c: 62.675Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.144 0.142 0.18
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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Citations

4 citation in other articles

Structure-Based Optimization of Covalent, Small-Molecule Stabilizers of the 14-3-3σ/ERα Protein-Protein Interaction from Nonselective Fragments.
Konstantinidou et al. (2023)
3 more

1 mention without citation

Phosphorylation code of human nucleophosmin includes four cryptic sites for hierarchical binding of 14-3-3 proteins.
Kapitonova et al. (2024)

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