› 7oez

X-ray diffraction
2.48Å resolution

Leucine Aminopeptidase A mature enzyme in a complex with leucine

Released:
DOI: 10.2210/pdb7oez/pdb
PDB entry 7oez coloured by chain, front view.
PDB entry 7oez coloured by chain, side view.
PDB entry 7oez coloured by chain, top view.
Source organism: Aspergillus oryzae RIB40
Primary publication:
The role of propeptide-mediated autoinhibition and intermolecular chaperone in the maturation of cognate catalytic domain in leucine aminopeptidase.
Baltulionis G, Blight M, Robin A, Charalampopoulos D, Watson KA
J Struct Biol 213 107741 (2021)
PMID: 33989771

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174060 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Leucine aminopeptidase A Chain: A
Molecule details ›
Chain: A
Length: 301 amino acids
Theoretical weight: 32.78 KDa
Source organism: Aspergillus oryzae RIB40
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q2U1F3 (Residues: 77-377; Coverage: 84%)
Gene names: AO090011000052, lapA
Sequence domains: Peptidase family M28

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
5 bound ligands:
Ligand SO4 5 x SO4
Ligand ZN 2 x ZN
Ligand CL 1 x CL
Ligand EDO 5 x EDO
Ligand LEU 1 x LEU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P6322
Unit cell:
a: 154.1Å b: 154.1Å c: 87.83Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.16 0.157 0.215
Expression system: Komagataella pastoris

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Citations

1 review citation

Microbial proteases and their applications.
Song et al. (2023)
1 other article