› 7ogd

X-ray diffraction
1.95Å resolution

GTPase HRAS mutant D33K under ambient pressure

Released:
DOI: 10.2210/pdb7ogd/pdb
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PDB entry 7ogd coloured by chain, front view.
PDB entry 7ogd coloured by chain, side view.
PDB entry 7ogd coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Equilibria between conformational states of the Ras oncogene protein revealed by high pressure crystallography.
Girard E, Lopes P, Spoerner M, Dhaussy AC, Prangé T, Kalbitzer HR, Colloc'h N
OpenAccess logo Chem Sci 13 2001-2010 (2022)
PMID: 35308861

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133956 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTPase HRas, N-terminally processed Chain: A
Molecule details ›
Chain: A
Length: 166 amino acids
Theoretical weight: 18.89 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P01112 (Residues: 1-166; Coverage: 88%)
Gene names: HRAS, HRAS1
Sequence domains: Ras family

Ligands and Environments

3 bound ligands:
Ligand GNP 1 x GNP
Ligand MG 1 x MG
Ligand PGE 1 x PGE
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE ID27
Spacegroup: P3221
Unit cell:
a: 39.787Å b: 39.787Å c: 161.7Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.184 0.181 0.25
Expression system: Escherichia coli BL21

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Citations

5 citation in other articles

Delineating the RAS Conformational Landscape.
Parker et al. (2022)
4 more

1 mention without citation

Equilibria between conformational states of the Ras oncogene protein revealed by high pressure crystallography.
Girard et al. (2022)

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